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Replication cycle-coordinated change of the adenine nucleotide-bound forms of DnaA protein in Escherichia coli

✍ Scribed by Kurokawa, K.


Book ID
111746254
Publisher
Nature Publishing Group
Year
1999
Tongue
English
Weight
372 KB
Volume
18
Category
Article
ISSN
0261-4189

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Flow cytometry was used to study initiation of DNA replication in Escherichia coli K12 after induced expression of a plasmid-borne dnaA+ gene. When the dnaA gene was induced from either the plac or the lambda pL promoter initiation was stimulated, as evidenced by an increase in the number of origins