Functions of the DnaA protein of Escherichia coli in replication and transcription
β Scribed by Walter Messer; Wolfgang Seufert; Christoph Schaefer; Annette Gielow; Heidi Hartmann; Martina Wende
- Book ID
- 119119697
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 924 KB
- Volume
- 951
- Category
- Article
- ISSN
- 0167-4781
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Flow cytometry was used to study initiation of DNA replication in Escherichia coli K12 after induced expression of a plasmid-borne dnaA+ gene. When the dnaA gene was induced from either the plac or the lambda pL promoter initiation was stimulated, as evidenced by an increase in the number of origins
The in vivo role of the Escherichia coli protein DnaA in the replication of plasmid pBR322 was investigated, using a plasmid derivative carrying an inducible dnaA+ gene. In LB medium without inducer, the replication of this plasmid, like that of pBR322, was inhibited by heat inactivation of chromoso
Increased synthesis of DnaA protein, obtained with plasmids carrying the dnaA gene controlled by the heat inducible lambda pL promoter, stimulated initiation of replication from oriC about threefold. The overinitiation was determined both as an increase in copy number of a minichromosome and as an i