Flow cytometry was used to study initiation of DNA replication in Escherichia coli K12 after induced expression of a plasmid-borne dnaA+ gene. When the dnaA gene was induced from either the plac or the lambda pL promoter initiation was stimulated, as evidenced by an increase in the number of origins
Negative control of DNA replication by hydrolysis of ATP bound to DnaA protein, the initiator of chromosomal DNA replication in Escherichia coli
β Scribed by Mizushima, T.
- Book ID
- 111744849
- Publisher
- Nature Publishing Group
- Year
- 1997
- Tongue
- English
- Weight
- 271 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0261-4189
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