Coupling chromosomal replication to cell growth by the initiator protein DnaA in Escherichia coli
β Scribed by Qing Zhang; Hualin Shi
- Book ID
- 118138659
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 691 KB
- Volume
- 314
- Category
- Article
- ISSN
- 0022-5193
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Increased synthesis of DnaA protein, obtained with plasmids carrying the dnaA gene controlled by the heat inducible lambda pL promoter, stimulated initiation of replication from oriC about threefold. The overinitiation was determined both as an increase in copy number of a minichromosome and as an i
Flow cytometry was used to study initiation of DNA replication in Escherichia coli K12 after induced expression of a plasmid-borne dnaA+ gene. When the dnaA gene was induced from either the plac or the lambda pL promoter initiation was stimulated, as evidenced by an increase in the number of origins