Coupling the initiation of chromosome replication to cell size in Escherichia coli
โ Scribed by William D Donachie; Garry W Blakely
- Book ID
- 117764951
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 105 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1369-5274
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A new round of chromosomal replication of a temperature-sensitive initiation mutant (dnaC) of Escherichia coli was initiated synchronously by a temperature shift from a nonpermissive to a permissive condition in the presence of arabinosyl cytosine. Increased amounts of nascent DNA fragments with hom
It has been found that strains carrying mutations in the dnaA gene are unusually sensitive to COU, NAL or NOV, which are known to inhibit DNA gyrase activities. The delay in the initiation of chromosome replication after COU treatment has been observed in cells with chromosomes synchronized by amino