Growth of human immunodeficiency virus I in cultured cells in the absence of the CD4 antigen

✍ Scribed by N. K. Morrison; K. McCarthy; Professor C. A. Hart

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 605 KB
• Volume: 35
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890350308

✦ Synopsis

Abstract

The ability of a variety of epithelial, embryonal, placental, and neuronal cells to express the CD4 antigen and to be infected by human immuno deficiency virus 1 (HIV‐1) was examined. Only two (IMR‐32 and HeLa‐T4) expressed CD4 detectable by indirect immunofluorescence, and both were infectable by HIV‐1. Two others, a human laryngeal carcinoma (HEp‐2) and human colonic carcinoma (HT‐291, did not express CD4 antigen but were infectable by HIV‐I. Infection of the HEp‐2 cells was detectable four months (and 20 serial passages) later. Infection of HEp‐2 cells was not inhibited by anti CD4 monoclonal anti‐ body but was by the lectin concanavalin A. These results suggest the presence of a receptor other than CD4 can be involved in HIV‐1 infection.