Human immunodeficiency virus type 1 p24 production and antigenic variation in tissue culture of isolates with various growth characteristics

✍ Scribed by Dr. Vivi-Anne Sundqvist; Jan Albert; Eva Ohlsson; Jorma Hinkula; Eva Maria Fenyö; Britta Wahren

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 561 KB
• Volume: 29
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890290305

✦ Synopsis

Two monoclonal antibodies (Mabs) reacting with different epitopes of the human immunodeficiency virus type 1 core protein p24 (HIV p24) were used either singly or in combination as tracers in enzyme-linked immunosorbent assays. The culture supernatant of 21 5 samples of peripheral blood mononuclear cells from 112 patients were measured for HIV p24 and reverse transcriptase (RT) activity during cultivation. One hundred forty-one cultures were positive for HIV p24 and 122 for RT after 32 days of cultivation. After 8-9 days, HIV p24 was detected in 50.4% and RT in 27.8% of the cultures later judged as HIV positive. Two patients seemed to have substrains of HIV-1 not reactive with one of the Mabs.