Anti-human immunodeficiency virus activity of 3,4,5-tricaffeoylquinic acid in cultured cells of lettuce leaves

✍ Scribed by Hirotoshi Tamura; Takashi Akioka; Koichi Ueno; Takeshi Chujyo; Katsu-ichiro Okazaki; Peter J. King; W. Edward Robinson Jr.

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 581 KB
• Volume: 50
• Category: Article
• ISSN: 1613-4125
• DOI: 10.1002/mnfr.200500216

✦ Synopsis

Abstract

3,4,5–Tricaffeoylquinic acid (TCQA) that is not found in intact plant of lettuce leaves was isolated from the cultured cells. The intact plant produced chicoric acid (dicaffeoyl tartaric acid: L‐CCA) as well as chlorogenic acid (3‐caffeoylquinic acid: 3‐CQA) as the major metabolites. After subculturing of the cells for 40 days, the amount of 3,4,5‐TCQA reached to 0.14 mg/g fresh weight. The inhibitory effect of 3,4,5‐TCQA for human immunodeficiency virus (HIV) Type 1 integrase was assayed. Anti‐HIV activity using HIV and MT‐2 cells was 1.15 μM and IC~50~ against HIV integrase was 0.063 μM whereas cell toxicity of this chemical was expressed as 5% death of all living cells to be 18.4 μM. The HIV inhibitory effect of 3,4,5‐TCQA was the highest in values among L‐CCA, and other dicaffeoylquinic acids. This data will provide a new possibility for creating a new drug design for HIV.