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Growth of human × human hybridomas in protein-free medium supplemented with ethanolamine

✍ Scribed by S.P.C. Cole; E.H. Vreeken; S.E.L. Mirski; B.G. Campling

Book ID
118900595
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
497 KB
Volume
97
Category
Article
ISSN
0022-1759

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✍ Hiroshi Shinmoto; Shun'ichi Dosako 📂 Article 📅 1993 🏛 Springer Netherlands 🌐 English ⚖ 175 KB

Five mouse-human hybridoma cell lines secreting human IgM class monoclonal antibodies were examined for possible adaptation to a protein free medium. Four were successfully adapted to the medium. The adapted cell lines could be cultured for more than two months without any changes in their cell grow