Continuous hybridoma culture in a low-protein serum-free medium supplemented with liposomes
✍ Scribed by Adèle Martial; Isabelle Gaillard; Jean-Marc Engasser; Annie Marc
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 627 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0141-0229
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract The steady‐state metabolic parameters for a hybridoma cell line have been determined in continuous suspension–perfusion culture over a wide range of perfusion rates and cell bleed rates. Significant increases in viable cell concentrations and volumetric productivities were achieved at h
The murine B-lymphocyte hybridoma, CC9C10 was grown at steady state under serum-free conditions in continuous culture at dissolved oxygen (DO) concentrations in the range of 10% to 150% of air saturation. Cells could be maintained within this range at high viability in a steady state at a dilution r