𝔖 Bobbio Scriptorium
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Antibody production by human × human hybridomas in serum-free medium

✍ Scribed by S.P.C. Cole; E.H. Vreeken; J.C. Roder

Book ID
118899752
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
429 KB
Volume
78
Category
Article
ISSN
0022-1759

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Stimulation of monoclonal antibody produ
Stimulation of monoclonal antibody production by human-human hybridoma cells with an elevated concentration of potassium or sodium phosphate in serum-free medium
✍ S. Sato; H. Murakami; T. Sugahara; T. Ikegami; K. Yamada; H. Omura; S. Hashizume 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 325 KB

Potassium or sodium phosphate was found to stimulate the production of human monoclonal antibody by human-human hybridoma HB4C5. The addition of 15 mM Na-phosphate (pH 7.4) into serum-free culture medium increased the antibody production up to 4-fold, when seeded at cell density of 1 x 10 5 cells/ml