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Adaptation of mouse-human hybridomas to a protein free medium

✍ Scribed by Hiroshi Shinmoto; Shun'ichi Dosako

Publisher
Springer Netherlands
Year
1993
Tongue
English
Weight
175 KB
Volume
15
Category
Article
ISSN
0141-5492

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✦ Synopsis

Five mouse-human hybridoma cell lines secreting human IgM class monoclonal antibodies were examined for possible adaptation to a protein free medium. Four were successfully adapted to the medium. The adapted cell lines could be cultured for more than two months without any changes in their cell growth abilities.

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A human-human hybridoma HB4CS secreting human IgM class monoclonal antibody was examined for possible adaptation to a protein-free medium. HB4C5 could be cultured for more than two months in a protein-free medium without any significant changes in antibody production.