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Protein-free culture of a human-human hybridoma HB4C5

✍ Scribed by Hiroshi Shinmoto; Masuko Kobori; Tojiro Tsushida; Kazuki Shinohara

Publisher
Springer-Verlag
Year
1994
Tongue
English
Weight
113 KB
Volume
8
Category
Article
ISSN
0951-208X

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✦ Synopsis

A human-human hybridoma HB4CS secreting human IgM class monoclonal antibody was examined for possible adaptation to a protein-free medium.

HB4C5 could be cultured for more than two months in a protein-free medium without any significant changes in antibody production.

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Five mouse-human hybridoma cell lines secreting human IgM class monoclonal antibodies were examined for possible adaptation to a protein free medium. Four were successfully adapted to the medium. The adapted cell lines could be cultured for more than two months without any changes in their cell grow

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