𝔖 Bobbio Scriptorium
✦   LIBER   ✦

High-Density culture of mouse–human hybridoma in serum-free defined medium

✍ Scribed by Yoshiharu Takazawa; Michiyuki Tokashiki; Hiroki Murakami; Koji Yamada; Hirohisa Omura


Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
443 KB
Volume
31
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Fed-batch culture of hybridoma cells in
✍ Li Zhang; Hong Shen; Yuanxing Zhang 📂 Article 📅 2004 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 180 KB

## Abstract The achievement of high cell density and monoclonal antibody concentration depends on understanding cell metabolism and the control of cell culture. A model of cell metabolism and monoclonal antibody production in hybridoma cell culture is presented. Fed‐batch culture in serum‐free medi

Effect of serum-free and serum-containin
✍ Nathalie Lambert; Otto-W. Merten 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 568 KB

BiP, GRP94 and PDI, three endoplasmic reticulum (ER) based proteins are involved in the maturation of secretory proteins and might represent a bottleneck in the secretory pathway of monoclonal antibodies (MAB). With the three hybridoma cell lines tested, MAB production kinetics were significantly in

The effect of dissolved oxygen on the me
✍ D. C. H. Jan; D. A. Petch; N. Huzel; M. Butler 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 184 KB 👁 2 views

The murine B-lymphocyte hybridoma, CC9C10 was grown at steady state under serum-free conditions in continuous culture at dissolved oxygen (DO) concentrations in the range of 10% to 150% of air saturation. Cells could be maintained within this range at high viability in a steady state at a dilution r

High cell density culture of metabolical
✍ Fulai Wang; Sang Yup Lee 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 67 KB 👁 2 views

A recombinant Escherichia coli strain XL1-Blue harboring a stable high-copy-number plasmid pSYL107 containing the Alcaligenes eutrophus polyhydroxyalkanoate biosynthesis genes and the Escherichia coli ftsZ gene was employed for the production of poly(3hydroxybutyrate) (PHB) by fed-batch culture in a

Differential expression of voltage-gated
✍ Francisco J. Urbano; Felipe Sierra; Jose M. Velasco; Washington Buño 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 152 KB

Voltage-gated Ca 21 conductances were investigated with the whole-cell patch-clamp technique-either using Ca 21 or Ba 21 as charge carriers-in NB69 human neuroblastoma cells plated in ''defined'' serum-free (DM) and in ''astroglial-conditioned'' media (CM). Cells expressed the microtubule associated