Madin Darby canine kidney cells can grow in synthetic medium supplemented with 5 factors ~ insulin. transferrin, prostaglandin E , , hydrocortisone and triiodothyronine ~-as a seruni substitute. These 5 factors permit growth for one month in the absence of serum, and a growth rate equivalent t o tha
Growth and Maintenance of HeLa Cells in Serum-Free Medium Supplemented with Hormones
β Scribed by Sharon E. Hutchings and Gordon H. Sato
- Book ID
- 123654929
- Publisher
- National Academy of Sciences
- Year
- 1978
- Tongue
- English
- Weight
- 1001 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/67895
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π SIMILAR VOLUMES
The procedure of analyzing hormone and growth factor requirements for the growth of MPC-I I cells and of developing a serum-free medium for this cell line has been described. In this medium, MPC-I I cells grow as fast as in serum-supplemented medium, up to 50 generations. MPC-I I cells grown in ser
Bovine milk contains growth promoting factors that stimulate DNA synthesis and cell division in confluent monolayers of quiescent Balb/c 3T3 cells. The growth factor activity was highest in colostrum obtained within 24 hours after birth of a calf. Samples of milk obtained 3 2 hours and 60 hours afte