Gm typing by enzyme-linked immunosorbent assay (ELISA)

✍ Scribed by Kishda, T. ;Tamaki, Y.

• Publisher: Springer-Verlag
• Year: 1984
• Tongue: English
• Weight: 270 KB
• Volume: 93
• Category: Article
• ISSN: 1437-1596
• DOI: 10.1007/bf00202980

✦ Synopsis

A solid-phase EL1SA for Gm typing is described. A mixture of anti-Gm serum (or monoclonal anti-Gin antibody) and test serum was incubated in microtiter wells coated with IgG or its fragments of appropriate Gm type. After washing of the wells, the bound antibody was detected with peroxidase-labeled second antibody. The Glm(3), G3m( 16), and G3m(21) antigens could be identified by this technique. Since some of the human anti-Gm sera and anti-Rh0 sera required for the conventional hemagglutinationinhibition method are hard to obtain, the ELISA system using anti-Gm antibodies and no anti-Rh0 sera may serve as an alternative to the conventional method.