Enzyme linked immunosorbent assay (ELISA) for bancroftian filariasis
β Scribed by P. Kaliraj; S.N. Ghirnikar; B.C. Harinath
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 228 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0035-9203
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W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub