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Enzyme-linked immunosorbent assay (ELISA) for mumps virus antibodies

✍ Scribed by Therese Popow-Kraupp


Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
558 KB
Volume
8
Category
Article
ISSN
0146-6615

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Enzyme-linked immunosorbent assay (ELISA
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## Abstract An ELISA for the detection of mumps virus‐specific IgG and IgM antibodies was developed. Three different antigen preparations were compared. Equally good results were obtained with virus concentrated by ultracentrifugation and with virus that was further purified by sucrose gradient cen

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W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub