𝔖 Bobbio Scriptorium
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Enzyme-linked immunosorbent assay (ELISA) in the paracoccidioidomycosis

✍ Scribed by Z. Pires Camargo; J. L. Guesdon; E. Drouhet; L. Improvisi

Publisher
Springer Netherlands
Year
1984
Tongue
English
Weight
471 KB
Volume
88
Category
Article
ISSN
0301-486X

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✦ Synopsis

An enzyme-linked immunosorbent assay (ELISA) for detection and quantification of antibodies anti-Paracoccidioides brasiliensis is described. Polystyrene plates have been used as solid phase to absorb P. brasiliensis metabolic yeast phase antigen. Twenty sera of proven paracoccidioidomycosis, 11 of histoplasmosis due Histoplasma capsulatum, 20 of aspergillosis and 20 human normal sera were tested. Ninety-five percent of the paracoccidioidomycosis sera had O.D. superior to 0.150 (from 0.163 to 2.650) at 1/400 serum dilution. ELISA assay was compared with counterimmunoelectrophoresis and erythro-immunoassay tests; a correlation was observed only with erythro-immunoassay. ELISA test should give new perspectives for the serodiagnosis of paracoccidioidomycosis.

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