Replacement of the regulatory sequence of the Bacillus amyloliquefaciens a-amylase gene (AMYI) by the yeast alcohol deh of extracellular a-amylase drogenase gene promoter (ADCZp) resulted in increased levels glucoamylase synthesis by t R ro uction in Saccharomyces cerevuiae. Negative regulation of J
Glucoamylase and α-amylase production by immobilizedAspergillus niger
✍ Scribed by Gao-Xiang Li; Yu-Yen Linko; P. Linko
- Publisher
- Springer Netherlands
- Year
- 1984
- Tongue
- English
- Weight
- 405 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0141-5492
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✦ Synopsis
AspergiZius niger mycelia or spores were imnobilized in calcium alginate gel beads and employed for production of glucoamylase and a-amylase by repeated batch process. The immobilized mycelium produced lower enzyme activities than imnobilized spores germinated in a growth medium and subsequently cultured in an enzyme production medium. In repeated batch experiments, free cells could be used for only 4 4-day batches, whereas with immobilized spores at least 11 4-day batches with a gradual increase in enzyme activi ties in each successive batch were possible. The activity ratio of glucoamylase and a-amylase produced was altered by immobilization.
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