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Optimization of α-amylase and glucoamylase production by recombinant strains ofSaccharomyces cerevisiae

✍ Scribed by Vincenzo S. D'Aguanno; Isak S. Pretorius

Publisher
Springer Netherlands
Year
1994
Tongue
English
Weight
420 KB
Volume
16
Category
Article
ISSN
0141-5492

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✦ Synopsis

Replacement of the regulatory sequence of the Bacillus amyloliquefaciens a-amylase gene (AMYI) by the yeast alcohol deh of extracellular a-amylase drogenase gene promoter (ADCZp) resulted in increased levels glucoamylase synthesis by t R ro uction in Saccharomyces cerevuiae. Negative regulation of J e STAlO-encoded repressor was alleviated by replacing the native STA2 gene promoter from S. cerevisiae var. diastaticus with ADCI . Enhanced degradation of starch was achieved when the modified versions of the AMY1 and ,!!?A2 genes were introduced jointly into S. cerevisiae.

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