GLC determination of quinidines in human plasma

✍ Scribed by J. L. Valentine; Patricia Driscoll; Ellen L. Hamburg; Everett D. Thompson

• Publisher: John Wiley and Sons
• Year: 1976
• Tongue: English
• Weight: 373 KB
• Volume: 65
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600650120

✦ Synopsis

Human plasma was made alkaline and extracted with methylene chloride. To the extract was added the internal standard, cinchonine, followed by evaporation to dryness. The resultant residue was dissolved in a methanolic solution containing trimethylanilinium hydroxide. This solution was assayed by GLC for quinidines (quinidine and hydroquinidine). Evaluation of the method over a 0.5-10-pg/ml range in human plasma gave an overall precision and accuracy of &4.5% (RSD and RE). Plasma of several patients was analyzed by the present method as well as by a fluorometric method for the level of quinidines. Results from the two methods were comparable.

Keyphrases Quinidine-GLC analysis in human plasma, compared to fluorometric analysis 0 Hydroquinidine-GLC analysis in human plasma, compared to fluorometric analysis 0 GLCanalysis, quinidine and hydroquinidine in human plasma, compared to fluorometric method 0 Antiarrhythmic agents-quinidine and hydroquinidine, GLC analysis in human plasma, compared to fluorometric analysis Vortex. MethElute, Pierce Chemical C o Packard 419.