Genetic mapping of the polycystic kidney gene,pcy, on mouse chromosome 9
β Scribed by Shizuko Nagao; Tomomasa Watanabe; Noboru Ogiso; Tohru Marunouchi; Hisahide Takahashi
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 643 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0006-2928
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A human cDNA for biglycan (BGN) has recently been mapped to proximal Xq28. We have mapped the murine locus, Bgn, approximately 50 kb distal to DXPas8, using a combination of genetic mapping in an interspecific backcross of B6CBA-AW-J/A -Bpa x Mus spretus and physical mapping using pulsed field gel e
## Mouse Locus name: T cell-specific transcription factor 7 Locus symbol: Tcf7 Map position: centromere-D11Sell-2,6 + 1.5 cM-Tcf7/IL3-1.8 +\_ 1.2 cM-Gtrl-telomere Method of mapping: (C3H/HeJ-g/d x Mus spretus)F 1 X C3H/HeJgld interspecific backcross mice [1,2]. Haplotype analysis is shown in Fig.
## Method of mapping: 113 animals from the backcross (DP-BB β’ DR-BB)F 1 β’ DP-BB were analyzed. Molecular reagent: Primer sequences used for PCR were a sense primer, 5'-CTCCCAGCTGTGGATGGGTTC-3' (nucleotide position 3134 to 3154 of mouse Hoxall homeobox gene), and an anti-sense primer, 5'-CCAGAACAGC