Genetic mapping of the tumor-associated mucin 1 gene on mouse Chromosome 3
โ Scribed by S. F. Kingsmore; A. P. Spicer; S. J. Gendler; M. F. Seldin
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 329 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0938-8990
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โฆ Synopsis
Mouse
Locus name: T cell-specific transcription factor 7 Locus symbol: Tcf7 Map position: centromere-D11Sell-2,6 + 1.5 cM-Tcf7/IL3-1.8 +_ 1.2 cM-Gtrl-telomere Method of mapping: (C3H/HeJ-g/d x Mus spretus)F 1 X C3H/HeJgld interspecific backcross mice [1,2]. Haplotype analysis is shown in Fig. 1.
๐ SIMILAR VOLUMES
A human cDNA for biglycan (BGN) has recently been mapped to proximal Xq28. We have mapped the murine locus, Bgn, approximately 50 kb distal to DXPas8, using a combination of genetic mapping in an interspecific backcross of B6CBA-AW-J/A -Bpa x Mus spretus and physical mapping using pulsed field gel e
## Molecular reagents: The downstream and upstream primers, m2pl2A and m2pl2B, had the following sequences: 5'-TTC AGC GTC TCT AAT ACC AAA TCT-3' and 5'-AGC CAC CAT AAA CCA TAA ACA CTT-3", respectively. These primers cover a predicted product of 157 bp.
## Method of mapping: 113 animals from the backcross (DP-BB โข DR-BB)F 1 โข DP-BB were analyzed. Molecular reagent: Primer sequences used for PCR were a sense primer, 5'-CTCCCAGCTGTGGATGGGTTC-3' (nucleotide position 3134 to 3154 of mouse Hoxall homeobox gene), and an anti-sense primer, 5'-CCAGAACAGC