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Genetic mapping of opioid binding protein gene(s) to mouse Chromosome 9

✍ Scribed by Anuradha Chakraborti; David L. Lippman; Horace H. Loh; Christine A. Kozak; Nancy M. Lee

Publisher: Springer-Verlag
Year: 1993
Tongue: English
Weight: 483 KB
Volume: 4
Category: Article
ISSN: 0938-8990
DOI: 10.1007/bf00352235

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## Molecular reagents: The downstream and upstream primers, m2pl2A and m2pl2B, had the following sequences: 5'-TTC AGC GTC TCT AAT ACC AAA TCT-3' and 5'-AGC CAC CAT AAA CCA TAA ACA CTT-3", respectively. These primers cover a predicted product of 157 bp.

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## Method of mapping: 113 animals from the backcross (DP-BB • DR-BB)F 1 • DP-BB were analyzed. Molecular reagent: Primer sequences used for PCR were a sense primer, 5'-CTCCCAGCTGTGGATGGGTTC-3' (nucleotide position 3134 to 3154 of mouse Hoxall homeobox gene), and an anti-sense primer, 5'-CCAGAACAGC