We studied the repair of double-strand breaks (DSB) in plasmid DNA introduced into haploid cells of the yeast Saccharomyces cerevisiae. The efficiency of repair was estimated from the frequency of transformation of the cells by an autonomously replicated linearized plasmid. The frequency of "lithium
Genetic analysis of the yeast NUD1 endo-exonuclease: a role in the repair of DNA double-strand breaks
โ Scribed by Benyam Asefa; Paul Kauler; Denis Cournoyer; Shirley Lehnert; T. Y.-K. Chow
- Publisher
- Springer-Verlag
- Year
- 1998
- Tongue
- English
- Weight
- 122 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0172-8083
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A model is described for the repair of induced double-strand breaks in DNA which involves a proposed heteroduplex intermediate and normally occurring repair enzymes. The model predicts that this repair can lead to non-reciprocal and/or reciprocal recombination. The evidence which has been evaluated
The repair of double-strand gaps (DSGs) in the plasmid DNA of radiosensitive mutants of Saccharomyces cerevisiae has been analyzed. The proportion of repair events that resulted in complete plasmid DNA DSG recovery was close to 100% in Rad+ cells. Mutation rad55 does not influence the efficiency and