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Genetic control of plasmid DNA double-strand gap repair in yeast,Saccharomyces cerevisiae

โœ Scribed by V. M. Glaser; A. V. Glasunov; G. G. Tevzadze; J. R. Perera; S. V. Shestakov

Publisher
Springer-Verlag
Year
1990
Tongue
English
Weight
740 KB
Volume
18
Category
Article
ISSN
0172-8083

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โœฆ Synopsis

The repair of double-strand gaps (DSGs) in the plasmid DNA of radiosensitive mutants of Saccharomyces cerevisiae has been analyzed. The proportion of repair events that resulted in complete plasmid DNA DSG recovery was close to 100% in Rad+ cells. Mutation rad55 does not influence the efficiency and preciseness of DSG repair. The mutant rad57, which is capable of recombinational DNA DSB repair, resulted in no DSG recovery. Mutation rad53 substantially inhibits the efficiency of DSG repair but does not influence the precision of repair. Plasmid DNA DSG repair is completely blocked by mutations rad50 and rad54.

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