The formation and repair of double-strand breaks induced in DNA by MMS was studied in haploid wild type and MMS-sensitive rad6 mutant strains of Saccharomyces cerevisiae with the use of the neutral and alkaline sucrose sedimentation technique. A similar decrease in average molecular weight of double
โฆ LIBER โฆ
A rapid method to monitor repair and mis-repair of DNA double-strand breaks by using cell extracts of the yeast Saccharomyces cerevisiae
โ Scribed by Bhavanath Jha; F. Ahne; Manfred Kistler; Christian Klaus; Friederike Eckardt-Schupp
- Publisher
- Springer-Verlag
- Year
- 1998
- Tongue
- English
- Weight
- 50 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0172-8083
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An episomal DNA vector (YpJA18), encoding two selectable recombinant yeast genes (TRP1, URA3), was constructed to assess the fidelity of DNA repair in haploid repair-competent (RAD) wild-type yeast and several radiation-sensitive mutants. Either a DNA double-strand break (DSB) or a double-strand gap