Genetic analysis of the mobilization of the non-conjugative plasmid Clo DF13
โ Scribed by van de Pol, H. ;Veltkamp, E. ;Nijkamp, H. J. J.
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 988 KB
- Volume
- 160
- Category
- Article
- ISSN
- 0026-8925
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The entire region required for mobilization of the non-conjugative plasmid RSF1010 has been cloned into a mobilization-deficient pBR322 derivative. The segment of DNA cloned was approximately 1.8 kb and included the origin of conjugal DNA transfer (oriT). The DNA sequence of the mobilization region
The oriT site of the broad host-range multicopy IncQ plasmid RSF1010 was cloned onto the 2.2 kb pBR322-derived vector pED825. By successive subcloning and construction of deletions, the oriT region was localised on an 80-88 bp segment of DNA. This segment was contained within the HaeII fragment of R
An ampicillin transposon Tn901 was used as a "mutagen" to isolate insertion mutants of the bacteriocinogenic plasmid Clo DF13. By combining the obtained heteroduplex and restriction maps of the Clo DF13::Tn901 plasmids (van Emboden et al., 1977b) with their polypeptide pattern in minicells, we were