Maintenance of the bacteriocinogenic plasmid Clo DF13 in Escherichia coli cells
โ Scribed by Hakkaart, Marcel J. J. ;Veltkamp, Eduard ;Niikamp, H. John J.
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 849 KB
- Volume
- 188
- Category
- Article
- ISSN
- 0026-8925
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An ampicillin transposon Tn901 was used as a "mutagen" to isolate insertion mutants of the bacteriocinogenic plasmid Clo DF13. By combining the obtained heteroduplex and restriction maps of the Clo DF13::Tn901 plasmids (van Emboden et al., 1977b) with their polypeptide pattern in minicells, we were
A class of F' plasmids, designated Fpoh+, was previously shown to be able to replicate extra-chromosomally on Hfr strains by virtue of carrying the specific site or region poh+ (permissive on Hfr) of the E. coli chromosome (Hiraga, 1975, 1976a). These plasmids were now found to replicate on E. coli
The gene expression of the Clo DF13 "replication region", located between 1.8% and 12% on the plasmid genome, was studied using newly constructed Clo DF13 insertion and deletion mutants. We were able to detect a Clo DF13 specified protein of 6 kilodaltons (kd) by electrophoretic analysis of plasmid