The entire region required for mobilization of the non-conjugative plasmid RSF1010 has been cloned into a mobilization-deficient pBR322 derivative. The segment of DNA cloned was approximately 1.8 kb and included the origin of conjugal DNA transfer (oriT). The DNA sequence of the mobilization region
Mobilization of the non-conjugative plasmid RSF1010: A genetic and DNA sequence analysis of the mobilization region
โ Scribed by Derbyshire, Keith M. ;Hatfull, Graham ;Willetts, Neil
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 35 KB
- Volume
- 209
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The oriT site of the broad host-range multicopy IncQ plasmid RSF1010 was cloned onto the 2.2 kb pBR322-derived vector pED825. By successive subcloning and construction of deletions, the oriT region was localised on an 80-88 bp segment of DNA. This segment was contained within the HaeII fragment of R
The origin of transfer (oriT) is the sequence within which conjugal transfer of plasmid DNA is initiated, and is absolutely required in cis for plasmid mobilization. We have cloned oriT from the 52 kb IncN plasmid R46 on a 600 bp fragment, and mapped the limits of the relevant sequence by deletion a