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Mobilization of the non-conjugative plasmid RSF1010: A genetic analysis of its origin of transfer

โœ Scribed by Derbyshire, Keith M. ;Willetts, Neil S.

Publisher
Springer
Year
1987
Tongue
English
Weight
35 KB
Volume
209
Category
Article
ISSN
0026-8925

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Mobilization of the non-conjugative plas
Mobilization of the non-conjugative plasmid RSF1010: A genetic analysis of its origin of transfer
โœ Derbyshire, Keith M. ;Willetts, Neil S. ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Springer ๐ŸŒ English โš– 836 KB

The oriT site of the broad host-range multicopy IncQ plasmid RSF1010 was cloned onto the 2.2 kb pBR322-derived vector pED825. By successive subcloning and construction of deletions, the oriT region was localised on an 80-88 bp segment of DNA. This segment was contained within the HaeII fragment of R

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Mobilization of the non-conjugative plasmid RSF1010: A genetic and DNA sequence analysis of the mobilization region
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The entire region required for mobilization of the non-conjugative plasmid RSF1010 has been cloned into a mobilization-deficient pBR322 derivative. The segment of DNA cloned was approximately 1.8 kb and included the origin of conjugal DNA transfer (oriT). The DNA sequence of the mobilization region

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The origin of transfer (oriT) of the conjugative plasmid R46: Characterization by deletion analysis and DNA sequencing
โœ Coupland, George M. ;Brown, Anthony M. C. ;Willetts, Neil S. ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Springer ๐ŸŒ English โš– 900 KB

The origin of transfer (oriT) is the sequence within which conjugal transfer of plasmid DNA is initiated, and is absolutely required in cis for plasmid mobilization. We have cloned oriT from the 52 kb IncN plasmid R46 on a 600 bp fragment, and mapped the limits of the relevant sequence by deletion a