Frequent loss of chromosome arm Ip DNA in parathyroid adenomas

✍ Scribed by Vincent L. Cryns; Su Min Yi; Hideki Tahara; Randall D. Gaz; Dr. Andrew Arnold

• Book ID: 102846827
• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 826 KB
• Volume: 13
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870130103

✦ Synopsis

Two molecular defects have been described in parathyroid adenomas: rearrangement and overexpression of the PRADllcyclin D I oncogene and allelic loss of chromosome I I DNA, often including the multiple endocrine neoplasia type I (MENI) putative tumor suppressor gene region. In an effort to identify additional parathyroid tumor suppressor genes, we examined 25 parathyroid adenomas for tumor-specific allelic loss of polymorphic DNA loci located near known or candidate tumor suppressor genes. Control leukocyte DNA from all 25 patients was heterozygous for I or more of the 9 chromosome I markers examined. Allelic loss at I or more of these informative loci on chromosome I was observed in 10 of 25 (40%) adenomas. Although many tumors lost extensive regions on chromosome I, all but one of these tumors had allelic loss of distal I p (I p32-pter); four tumors also lost loci on I q. Allelic loss at I I q I 3, the site of the MEN I gene, was detected in 5 of 2 I (24%) informative cases, including 3 with Ip loss. In contrast, allelic loss was rarely observed at loci on 99 and lop and was not observed at loci on 3p, 3q. 4p, 5q, l2q, 14q, 18q, 22q, or Xp. In summary, clonal allelic loss of loci on chromosome arm I p is a frequent feature of parathyroid adenomas, implying that inactivation of a tumor suppressor gene(s) on Ip commonly contributes to their pathogenesis. Genes Chromosom Cancer 13:9-17 (1995). 0 1995 Wiley-Lis, IK.