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Fractionation of yeast invertase isozymes and determination of enzymatic activity in sodium dodecyl sulfate-polyacrylamide gels

✍ Scribed by Peter Babczinski

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
770 KB
Volume
105
Category
Article
ISSN
0003-2697

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✦ Synopsis

Three invertase isozymes extracted from derepressed yeast cell membranes with 1% deoxycholate (0.5 mg/mg protein) were separated on sodium dodecyl sulfate (0.25%)-polyacrylamide gels (

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Quantitative determination of overlapped
Quantitative determination of overlapped proteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis
✍ Yasuhiro Kitazoe; Masanobu Miyahara; Nobuaki Hiraoka; Hisashi Ueta; Kozo Utsumi 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 567 KB

A method for resolving an overlapped polypeptide pattern of sodium dodecyl sulfate-polyacrylamide gel electrophoresis was described. The procedure was essentially a Gaussian fitting using the least squares method, and could resolve more than 20 overlapped components simultaneously. The applicability