Quantitative determination of overlapped proteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis

✍ Scribed by Yasuhiro Kitazoe; Masanobu Miyahara; Nobuaki Hiraoka; Hisashi Ueta; Kozo Utsumi

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 567 KB
• Volume: 134
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90300-7

✦ Synopsis

A method for resolving an overlapped polypeptide pattern of sodium dodecyl sulfate-polyacrylamide gel electrophoresis was described. The procedure was essentially a Gaussian fitting using the least squares method, and could resolve more than 20 overlapped components simultaneously. The applicability to overlapped and shouldered patterns was evaluated using practical electrophoretic data with varying amounts of mitochondrial samples. The relative contents of respective polypeptide components gave a good agreement regardless of the loaded amounts.