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Activity staining of glutathione peroxidase after electrophoresis on native and sodium dodecyl sulfate polyacrylamide gels

✍ Scribed by Chien-Liang Lin; Hsien-Jung Chen; Wen-Chi Hou


Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
948 KB
Volume
23
Category
Article
ISSN
0173-0835

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A nonradioactive method is developed to detect DNA polymerase activity after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis containing gapped DNA as template. The technique is based on the use of digoxigenin- or biotin-labeled deoxynucleotides during DNA synthesis, and their detecti