A solid-phase protein assay (SPA) for the determination of protein concentrations in the nanogram range is described. The technique is based on biotinylation of immobilized protein on the solid phase of a microtiter plate and quantitation of the protein-biotin complexes by peroxidase-coupled avidin.
✦ LIBER ✦
Fluorometric assay of proteins in the nanogram range
✍ Scribed by Peter Böhlen; Stanley Stein; Wallace Dairman; Sidney Udenfriend
- Book ID
- 117042807
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 642 KB
- Volume
- 155
- Category
- Article
- ISSN
- 0003-9861
No coin nor oath required. For personal study only.
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Visual detection limit Staining reagent (ng biotinylated DNA per Time for color Stability of composition slot blot) Color of stain development stain color Complete reagent 11 Yellow-brown 10-15 min 4 weeks Complete reagent without 11 Yellow-brown 10-15 min 4 weeks luminol Complete reagent without 25
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