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A Red-Dot-Blot Protein Assay Technique in the Low Nanogram Range

✍ Scribed by Tülin Morçöl; Anuradha Subramanian

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
173 KB
Volume
270
Category
Article
ISSN
0003-2697

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✍ M.W. Sulter; G.J. Kloosterhuis; P.J. Coenraads; H.H. Pas 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 246 KB

A solid-phase protein assay (SPA) for the determination of protein concentrations in the nanogram range is described. The technique is based on biotinylation of immobilized protein on the solid phase of a microtiter plate and quantitation of the protein-biotin complexes by peroxidase-coupled avidin.