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A fluorometric assay for trehalose in the picomole range

✍ Scribed by Petronia Carillo, Regina Feil, Yves Gibon, Namiko Satoh-Nagasawa…


Book ID
120695583
Publisher
BioMed Central
Year
2013
Tongue
English
Weight
630 KB
Volume
9
Category
Article
ISSN
1746-4811

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Fluorescence of the thiobarbituric acid reaction product of periodatecleaved sialic acid is readily detected by excitation at 550 nm and analysis of emitted light at 570 nm. A fluorometric microassay is described which is 500-fold more sensitive than the usual spectrophotometric procedures and readi