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Fluorometric assay of sialic acid in the picomole range: A modification of the thiobarbituric acid assay

✍ Scribed by K.S. Hammond; D.S. Papermaster

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
345 KB
Volume
74
Category
Article
ISSN
0003-2697

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✦ Synopsis

Fluorescence of the thiobarbituric acid reaction product of periodatecleaved sialic acid is readily detected by excitation at 550 nm and analysis of emitted light at 570 nm. A fluorometric microassay is described which is 500-fold more sensitive than the usual spectrophotometric procedures and readily detects 10 ng (32 pmol) of siahc acid. Contamination by 2-deoxyribose from cellular material is easily detected by shifts of excitation maxima below 550 nm.

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