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Fluorometric assay for determining epoxide hydrolase activity

โœ Scribed by Kai Doderer; Rolf D. Schmid

Book ID
111573544
Publisher
Springer Netherlands
Year
2004
Tongue
English
Weight
83 KB
Volume
26
Category
Article
ISSN
0141-5492

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A rapid, continuous, and highly sensitive fluorescence assay is described for the measurement of epoxide hydrase activity. The method is based on the large differences between the fluorescence spectra of certain K-region arene oxides and their corresponding transdihydrodiols. Enzymatic hydration of

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Two convenient and sensitive continuous spectrophotometric assays for cytosolic epoxide hydrolase are described. The assays are based on the differences in the ultraviolet spectra of the epoxide substrates and their diol products. The hydrolysis of 1,2-epoxy-1-(p-nitrophenyl)pentane (ENP5) is accomp