๐”– Bobbio Scriptorium
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Continuous fluorometric assay of epoxide hydrase activity

โœ Scribed by Patrick M. Dansette; Garrett C. DuBois; Donald M. Jerina

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
478 KB
Volume
97
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A rapid, continuous, and highly sensitive fluorescence assay is described for the measurement of epoxide hydrase activity. The method is based on the large differences between the fluorescence spectra of certain K-region arene oxides and their corresponding transdihydrodiols. Enzymatic hydration of K-region arene oxides of phenanthrene, pyrene, benzo[a]pyrene, and 7,12-dimethyibenzo[a]anthracene was studied. The assay was most sensitive with benzo[a]pyrene-4,Soxide as substrate. With 10 /.LM benzo[a]pyrene4,Soxide, enzymatic rates of 30 pmol of dihydrodioYmin/mg of protein are three to five times those of the blank without enzyme. The fluorometric method described has been used to study site-directed inhibitors of epoxide hydrase and the stereoselective hydration of racemic arene oxides.

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