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A Continuous Fluorometric Assay of Myristoyl-Coenzyme A:Protein N-Myristoyltransferase

โœ Scribed by Cynthia R. Pennise; Nafsika H. Georgopapadakou; R.Dale Collins; Nilsa R. Graciani; David L. Pompliano

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
49 KB
Volume
300
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Site-specific attachment of hydrophobic moieties to proteins (fatty acid acylation and prenylation) is a functionally essential modification involved in intracellular signaling pathways (1-3). Study of the transferase enzymes that catalyze these modifications is complicated by troublesome single point methods for assaying their activity. We have developed a continuous fluorometric assay for one of these enzymes, myristoyl-CoA 2 :protein N-myristoyltransferase (Nmt), useful for high-throughput assays and enzyme kinetics studies.

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