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A spectrophotometric method to assay epoxide hydrolase activity

✍ Scribed by Tej Bhatnagar; Kelath M Manoj; Jacques C Baratti


Book ID
117331096
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
122 KB
Volume
50
Category
Article
ISSN
0165-022X

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Continuous spectrophotometric assays for
✍ Roger N. Wixtrom; Bruce D. Hammock πŸ“‚ Article πŸ“… 1988 πŸ› Elsevier Science 🌐 English βš– 670 KB

Two convenient and sensitive continuous spectrophotometric assays for cytosolic epoxide hydrolase are described. The assays are based on the differences in the ultraviolet spectra of the epoxide substrates and their diol products. The hydrolysis of 1,2-epoxy-1-(p-nitrophenyl)pentane (ENP5) is accomp