Continuous spectrophotometric assays for
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Roger N. Wixtrom; Bruce D. Hammock
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Article
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1988
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Elsevier Science
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English
β 670 KB
Two convenient and sensitive continuous spectrophotometric assays for cytosolic epoxide hydrolase are described. The assays are based on the differences in the ultraviolet spectra of the epoxide substrates and their diol products. The hydrolysis of 1,2-epoxy-1-(p-nitrophenyl)pentane (ENP5) is accomp