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Fluorometric assay for pancreatic cholesterylester hydrolase

โœ Scribed by Anneli Joutti; Leila Kotama; Jorma A. Virtanen; Paavo K.J. Kinnunen


Book ID
107737066
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
270 KB
Volume
36
Category
Article
ISSN
0009-3084

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A fluorometric assay for intestinal peptidases has been developed. Amino acids liberated by hydrolysis are estimated by use of L-amino-acid oxidase, peroxidase, and the fluorogenic reagent p-hydroxyphenylacetic acid, which yields a highly fluorescent compound on oxidation. During development of fluo