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Fluorimetric determination of carbamoyl phosphate

✍ Scribed by Ronald J.A. Wanders; Carlo Van Roermund; Cor Lof; Alfred J. Meijer

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
629 KB
Volume
129
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple fluorimetric assay for the determination of carbamoyl phosphate in tissue extracts is described. In the assay, production of ATP from carbamoyl phosphate and ADP by carbamate kinase is coupled to the formation of NADPH, using glucose, hexokinase, NADP+, and glucose-6-phosphate dehydrogenase. Production of NADPH in this system proved to be equal to the amount of carbamoyl phosphate present.

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