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FIA—fluorimetric determination of thiamine

✍ Scribed by J.Martinez Calatayud; C.Gomez Benito; D.Gaspar Gimenez

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
210 KB
Volume
8
Category
Article
ISSN
0731-7085

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✦ Synopsis

A flow injection-fluorimetric determination of thiamine is reported. The procedure is based on the oxidation of the analyte with potassium hexacyanoferrate(III) immobilized on an anionic exchange resin; the fluorescence is monitored in aqueous basic solution. Concentrations of the vitamin of 0.1-4 ppm have been determined; the relative standard deviation was 1.8%. The injection rate was 28 samples/h. The influence of other substances and the determination of the drug in a pharmaceutical formulation are also reported.

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