## Abstract The cellular concentration of phosphate, the main activator of phosphate activated glutaminase (PAG) is rather constant in brain and kidney. The enzyme activity, however, is modulated by a variety of compounds affecting the binding of phosphate, such as glutamate, calcium, certain long
Fluorimetric assay of phosphate-activated glutaminase
β Scribed by J. Butterworth; C.M. Yates
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 396 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0165-0270
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π SIMILAR VOLUMES
An assay method for glutaminase is described in which the rate of release of glutamate from glutamine is followed in a second step by means of two coupled reactions involving glutamate dehydrogenase and the phenazine methosulfate-catalyzed reduction of iodonitrotetrazolium chloride by NADH. The assa
In this paper we describe a fluorimetric assay for the measurement of long-chain acyl-CoA synthetase activity in rat liver postnuclear supernatants. The method is based upon the use of acyl-CoA oxidase which catalyzes the dehydrogenation of acyl-CoA esters to yield enoyl-CoA esters and H2O2. H2O2 su