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Flow-injection determination of starch and total carbohydrate with an immobilized glucoamylase reactor and pulsed amperometric detection

✍ Scribed by Larry A. Larew; David A. Mead Jr; Dennis C. Johnson

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 602 KB
Volume: 204
Category: Article
ISSN: 0003-2670
DOI: 10.1016/s0003-2670(00)86344-8

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✦ Synopsis

Starch and total carbohydrate are determined by using a flow-injection system comprised of an immobilized glucoamylase reactor followed by pulsed amperometric detection of the glucose produced. Glucoamylase, immobilized onto porous silica and packed into a short stainless steel column, is capable of nearly quantitative (98% ) conversion of the starch to glucose. The sensitivity of pulsed amperometric detection for soluble starch is increased 26-fold by first passing the starch through the immobilized glucoamylase reactor. This system is successfully used to determine total carbohydrate in beer samples. The method is simple, rapid and sensitive for starch.

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