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Flow cytometry of human spermatozoa

✍ Scribed by F. J. Otto; Ursula Hacker; J. Zante; J. Schumann; W. Göhde; M. L. Meistrich

Publisher
Springer
Year
1979
Tongue
English
Weight
273 KB
Volume
61
Category
Article
ISSN
1432-119X

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✦ Synopsis

Methods are given for the preparation and staining of human spermatozoa for flow cytometric DNA measurements. Using agents for the reductive cleavage of disulfide crosslinks and suitable proteolytic enzymes an effective decondensation of the sperm chromatin and a DNA-proportional uptake of fluorochromes is achieved. Thus reliable and precise measurements of the relative DNA content of human spermatozoa are possible and the two subpopulations of haploid spermatozoa can be distinguished according to the difference in their DNA content.

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## Contents Flow cytometric sorting technology was used to measure the difference in DNA content between X‐ and Y‐chromosome‐bearing spermatozoa in bucks. Spermatozoa were analysed by flow cytometry to characterize X‐ and Y‐chromosome‐bearing sperm populations and to quantify the DNA difference bet